Fig. 4: Development of dominant-metabolism compromised intermediate-chassis strategy to reconfigure ethanologen Z. mobilis into a lignocellulosic D-lactate producer. | Nature Communications

Fig. 4: Development of dominant-metabolism compromised intermediate-chassis strategy to reconfigure ethanologen Z. mobilis into a lignocellulosic D-lactate producer.

From: Paradigm of engineering recalcitrant non-model microorganism with dominant metabolic pathway as a biorefinery chassis

Fig. 4: Development of dominant-metabolism compromised intermediate-chassis strategy to reconfigure ethanologen Z. mobilis into a lignocellulosic D-lactate producer.The alternative text for this image may have been generated using AI.

a Construction and application of the 2,3-BDO intermediate-chassis for D-lactate production in Z. mobilis. b Process of constructing D-lactate producer of Z. mobilis using the 2,3-BDO intermediate-chassis. c Flask fermentation results of glucose consumption and D-lactate production of the recombinant D-lactate producer ZMB6 in different concentrations of glucose. d Fermentor results of glucose consumption and D-lactate production of the recombinant strain ZMB6 using corncob residue hydrolysate (CRH). Adh: alcohol dehydrogenase, AldC: acetolactate decarboxylase, Als: acetolactate synthase, Bdh: butanediol dehydrogenase, LmldhA (Ldh): lactate dehydrogenase from L. mesenteroides, Pdc (encoding by ZMO1360): pyruvate decarboxylase. Data are presented as mean ± s.e.m. (n = 2 biologically independent samples). Source data are provided as a Source Data file.

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