Fig. 2: Distinct projection targets of Cartpt+ and Oxtr+ SPNs.
From: Parallel labeled-line organization of sympathetic outflow for selective organ regulation in mice
Schematics illustrating the experimental design (a) and time line (b). AM adrenal medulla, ST sympathetic trunk. c Coronal section of the SC showing mCherry (mC) expression (magenta) along with Chat, Cartpt, or Oxtr expression (green). AAV8 hSyn-DIO-mCherry was used for labeling. The right graph displays quantification for efficiency (mC+/markers+) and specificity (marker+/mC+). d mC+ axons (magenta) of Chat+, Cartpt+, or Oxtr+ SPNs to DBH+ (green) areas in the CG/SMG, AM, and 8–12th ST. The right graph shows the percentage of mC+ axons in the DBH+ regions. Magenta signals in the ST outside the DBH+ area show axons in passage. *p < 0.05, **p < 0.01, and ***p < 0.001 by one-way analysis of variance (ANOVA) followed by a two-sided Welch’s t-test with Bonferroni correction. Insets (in c, d) show magnified and channel-separated images in the boxed area. Scale bars, 100 μm for low-magnification images (c, d), and 50 μm for magnified images. The number of animals (N) is indicated in blue within the panel. Data are shown as mean ± SD. Source data are provided as a Source Data file. For more data, see Supplementary Figs. 2 and 3.
