Fig. 8: Stromal modulation in T3M4-luci/PSC orthotopic xenografts.
From: Dual rectification of metabolism abnormality in pancreatic cancer by a programmed nanomedicine
a Schematic illustration of the treatment procedures and the grouping description. T3M4-luci/PSC orthotopic xenografted mice in different groups were treated with Saline (G1), CPL (G2), TPCA-1 (G3), T-CPL (G4), AsiG-CPL (G5), TPCA-1 + AsiG-CPL (G6), or T-AsiG-CPL (G7) (siG: 1.0 mg/kg; TPCA-1: 20 mg/kg). b Representative histological analysis with immunohistochemical (IHC) staining of α-SMA (brown), fibronectin (brown), and two-photon autofluorescence of collagen (green) in tumor sections. Scale bars, 50 μm. Quantitative analysis of the normalized α-SMA (c), fibronectin (d), and collagen (e) protein expression in tumors after different treatments (n = 3 mice). A total of 6 images for each group (two random fields for each tumor section, three mice per group) were analyzed semi-quantitatively. Schematic illustration (f) and three-dimensional reconstruction (g) of orthotopic pancreatic tumors at the starting and endpoint of the T-AsiG-CPL treatment, imaged with a two-photon microscope at 1150 nm excitation wavelength after intravenously injecting siG-Cy5 or T-AsiG-Cy5-CPL (1.0 mg/kg siG and 20 mg/kg TPCA-1) into the mice for 24 h, (n = 3 mice). Scale bar, 100 μm. h The corresponding intensity profile of the Cy5-labeled siG at the tumor depth of 0–120 μm (n = 3 mice). The data in (c-e) and (h) are presented as the mean ± SEM. One-way ANOVA with Bonferroni’s multiple comparisons test was used for statistical significance analysis. Source data are provided as a Source Data file.
