Table 3 Primers for Floxed PanK4 and HSA-Cre genotyping
From: Pantothenate kinase 4 controls skeletal muscle substrate metabolism
Name | Sequence 5´->3´ | Product size |
|---|---|---|
Pank4-A1 Loxp-F | GCCTTGAACCCAGATTCGTTAAGAACAG | WT:253 bp Mut:315 bp |
Pank4-A2 Loxp-R | GATGAGAGGAGAGAGTACCGATCGC | |
Pank4-Frt-F | CCTGGAATGTTCTAGATCCATTCCTC | WT:269 bp Mut:399 bp |
Pank4-Frt-R | CCAGACTTATATGGTGTCTGAGCTAC | |
Flp-F | TCAGCGATATTAAGAACGTTGATCCG | Mut: 224 bp |
Flp-R | TGAAGAATTGCCGGTCCTATTTACTCG | |
HSA-Cre-F | CGCATATGCTTCCATCCCCC | WT: - Cre: 350 bp |
HSA-Cre-R | AAGTAGAGTGGGGGTCAGCA | |
Pank4 sense | TAAGGAGCCTCCCAACCCACCA | WT: 124 bp Floxed: - |
Pank4 wt_anti | GAATGTTCTAGATCCATTCCTCATGTCTC | |
Pank4 sense | TAAGGAGCCTCCCAACCCACCA | WT: - Floxed: 130 bp |
Pank4 flox_anti | GCTATACGAAGTTATTAGGTGGATCCGA | |
Cre sense | ACGGACAGAAGCATTTTCCAGGT | WT: - Cre: 100 bp |
Cre anti | CGGTCGATGCAACGAGTGATG |
