Fig. 1: osp-1 mutations enhance oxidative stress-induced neurodegeneration.
From: OSP-1 protects neurons from autophagic cell death induced by acute oxidative stress
a Schematic (top) and epifluorescence image (bottom) of a C. elegans animal expressing GFP (pmec-4::GFP) and KillerRed (pmec-4::KillerRed; not visible in the green channel) in mechanosensory neurons (ALML/R, PLML/R, AVM, and PVM). Scale bar 100 μm. b Representative epifluorescence images of an intact ALM neuron in a non-illuminated animal (control, top panel), and a missing (dead) ALM after illumination that activates the KillerRed-induced oxidative stress. Scale bar 100 μm. c Quantification of the ALM cell death phenotype in hermaphrodites and males. d Quantification of the ALM cell death phenotype in wild-type (wt) and vd60 mutant animals. Comparisons between groups were done using a two-tailed unpaired t-test. e Quantification of the ALM cell death phenotype in wt, and non-transgenic animals (-) and transgenic siblings (+) expressing a wild-type copy of osp-1 under its endogenous promoter in the vd60 background. Comparisons between groups were done using one-way ANOVA with a Tukey’s test for multiple comparisons. f Quantification of the ALM cell death phenotype in wt and osp-1(vd98) mutants. Comparisons between groups were done using a two-tailed unpaired t-test. In panels (c to f), bars represent mean values; N values are indicated in the graphs; error bars represent the standard error (SE) of proportion; KillerRed was activated using LED illumination.
