Fig. 8: Dynamic cell imaging results.

a Fluorescence spectral variations of 1AggI (c = 0.80 × 10–5 M) in cell culture medium with 0.05% DMSO. b The corresponding kinetic profiles of the conversion from 1AggI to 1AggII by monitoring the emission intensities at 550 and 600 nm. λex = 405 nm. CLSM images of the spontaneous transformation of 1AggI to 1AggII in A549 cells at (c–e) red channel (λex = 405 nm, λem = 600–700 nm) and (f–h) green channel (λex = 405 nm, λem = 500–599 nm) for 0, 15, and 30 min. Scale bar: 40 μm. Cell imaging was repeated at least three times with similar results.