Fig. 7: DEER analysis of IrtAB.

a Spin-labeling positions are shown between intracellular pair N305C^IrtB-S221C^IrtB (green) and extracellular pairs S51C^IrtB-S153C^IrtB (blue) or A362C^IrtA-T579C^IrtA (orange) for cryo-EM structures (IF and OF_occl conformation) or a homology model based on the OF_open TM287/288 structure. IrtA is colored teal, and IrtB is magenta, and cysteines are shown as spheres. Dotted lines connect the thiol-groups. b Distances between nitroxide spin-labels (MTSSL) were simulated based on cryo-EM structures (for IF and OF_occl conformation, shaded gray and red areas, respectively) or the homology model (shaded blue) shown in (a). Measured distance distributions are shown as solid lines. The distance distributions and their corresponding uncertainties (shown as gray-shaded areas around the curves corresponding to a 95 % confidence bound) were determined using a neural network analysis (ref. ⁵³).