Fig. 2: cyCONDOR workflow for data pre-processing and annotation.
From: Unveiling the power of high-dimensional cytometry data with cyCONDOR
a Schematic overview of the first steps of cyCONDOR analysis, from data ingestion to cell labeling. b Pseudobulk Principal Component Analysis (PCA) colored by experimental groups. c Heatmap showing mean marker expression for each samples, column order is defined by hierarchical clustering. d UMAP colored by experimental group. e UMAP colored according to Phenograph clustering. f UMAP colored according to cell type annotation and heatmap of mean marker expression for each cell type, color coding legend is shared for both. g UMAP visualization of SpectralFlow data colored by Phenograph clustering. h UMAP visualization of CyTOF data colored by Phenograph clustering. i UMAP visualization of CITE-seq data colored by Phenograph clustering. Created in BioRender. Bonaguro, L. (2024) https://biorender.com/o70s217.
