Fig. 4: Kidney dysfunction triggers apoptosis in GC B cells.
From: The survival of B cells is compromised in kidney disease
NP-specific GC B cells from the control and AAN spleen (n = 5) were flow sorted on day 12 post-immunization and subjected to RNA-Seq analysis. A Relative expression of significantly differentially expressed genes (q-value < 0.05) related to cell cycle, and B Over-representation pathway analysis of significantly downregulated genes in AAN GC B cells. C MFI of Ki67+ GC B cells [NP-KLH: Ctrl (5), AAI (7)]. D Cell cycle status of GC B cells was analyzed at day 12 post-immunization [NP-KLH: Ctrl (15), AAI (15)]. E Relative expression of significantly differentially expressed genes (q-value < 0.05) related to apoptosis and F Over-representation pathway analysis of upregulated genes in GC B cells from AAN mice. G NP+ and NP− GC B cells staining for active caspase3 expression (n = 12–14) at day 12 post-immunization [NP-KLH: Ctrl (14), AAI (12)]. Relative expression of significantly differentially expressed genes (q-value < 0.05) related to H Light zone (LZ), and I Dark zone (DZ) GC B cells. J Number of LZ centrocytes (CXCR4loCD86hi) and DZ centroblasts (CXCR4hiCD86lo) at day 12 post-immunization [NP-KLH: Ctrl (10), AAI (12)]. Each dot represents individual mice and data pooled from at least 2–3 experiments (C, D, G, and J). Data expressed as Mean ± SD. Statistical analyses by two-sided t-test (C, D, G, and J). C *P = 0.0228. D G0-G1 ***P = 0.0001, S ****P < 0.0001. G **P = 0.0023. J ****P < 0.0001 and ***P = 0.0003. Source data are provided as a Source Data file.
