Fig. 4: Substrate scope of SbzI and engineering the substrate selectivity of SbzI. | Nature Communications

Fig. 4: Substrate scope of SbzI and engineering the substrate selectivity of SbzI.

From: Structure-function analysis of carrier protein-dependent 2-sulfamoylacetyl transferase in the biosynthesis of altemicidin

Fig. 4: Substrate scope of SbzI and engineering the substrate selectivity of SbzI.

A Enzyme reaction of SbzI with different lengths of fatty acyl-SbzG substrates. B, C LC-MS profiles of the enzyme reaction products of (B) wild type SbzGI and (C) SbzGI L150IA variant with different lengths of fatty acyl-SbzG substrates. D Comparison of the activities of wild type SbzGI and SbzGI L150IA variant. E LC-MS profiles of the enzyme reaction products of SbzGI double variants toward the C12-acyl-SbzG substrate. All reactions were performed in triplicates. Data are presented as mean values, and the error bars indicate standard deviations (SD).

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