Fig. 1: A highly contiguous T. brucei genome assembly was generated with ultra-long nanopore reads.
From: Inter-chromosomal transcription hubs shape the 3D genome architecture of African trypanosomes
a An overview of the features and regions on the megabase chromosomes of the Lister 427 T. brucei assembly that have been modified. Allele ‘A’ and ‘B’ are shown on the top and bottom of each chromosome, respectively. Modified regions (gaps or collapsed regions, fully or partially corrected) are marked in blue. Regions with open gaps or collapsed repeats that could not be corrected and remained unmodified are marked in red. b Sizes of closed gaps (top) and change in size of corrected repeat regions (bottom). c Illustration of our strategy to confirm the correctness of the modifications made to the genome. Modified regions are considered corrected if there are at least 2 VPRs with less than 5 kb difference between the distance where the VPRs map on the assembly and their distance based on ONT read. d Correctness evaluation of modified regions in the new genome assembly (version 12).
