Fig. 4: Autophagy is activated during the HS recovery phase.
From: Stress granules sequester autophagy proteins to facilitate plant recovery from heat stress
a The 5-day-old EYFP-ATG8f plants were subjected to treatment at 22 °C (control) or 38 °C (HS) for 1 h, followed by heat-stressed plants recovered at 22 °C for 1 h, 3 h, 6 h, 9 h, and 12 h. The Meristem zone of indicated samples was observed using a confocal microscope. Scale bar = 10 μm. b Statistical analysis of the number of foci signals in (a). Data represent mean ± SD; n = 10. Ten different 50 × 50 μm2 areas in the meristem zone were used for colocalization analysis. c TEM images of the EYFP-ATG8f plant during the recovery phase. EYFP-ATG8f plants were subjected to HS treatment at 38 °C for 1 h, followed by recovery at 22 °C for 3 h or 9 h. Immuno-gold labeling with GFP antibody showing the location of EYFP-ATG8f. The magenta arrowheads indicate the GFP-antibody-coated gold particles. Scale bar = 500 nm. A, autophagosome; ER, endoplasmic reticulum; G, Golgi apparatus; M, mitochondria; V, vacuole. d The 5-day-old EYFP-ATG8f plants were subjected to treatment at 22 °C (control) or 38 °C (HS) for 1 h, followed by heat-stressed plants recovered at 1/2 MS liquid medium supplemented with 1 μM Conc A for 1 h, 3 h, 6 h, 9 h, and 12 h at 22 °C. The maturation zone of the roots from the indicated samples was observed using a confocal microscope. Scale bar = 10 μm. e Statistical analysis of the number of autophagosomes in the vacuole in (d). Data represent mean ± SD; n = 10. Ten different 50 × 50 μm2 areas in the maturation zone were used for analysis. Source data are provided as a Source Data file.
