Fig. 8: 3D mapping of mitochondria inside presynaptic compartments and the nano-organization of Brp in the adult fly brain. | Nature Communications

Fig. 8: 3D mapping of mitochondria inside presynaptic compartments and the nano-organization of Brp in the adult fly brain.

From: Rapid lightsheet fluorescence imaging of whole Drosophila brains at nanoscale resolution by potassium acrylate-based expansion microscopy

Fig. 8

a Two-color MIP image of GFP-labeled TH-GAL4 (green) and mCherry-labeled mitochondria (red) in the PRW region of the PKA-ExM fly brain. b Expanded slice view of the red box in (a) showing only mitochondria. The white arrow marked as identical presynaptic compartment in (a) and (b). Five independent measurements performed with similar results. c A tiled lightsheet image of the central complex of the re-PKA ExM fly brain, showing an expanded FB (yellow) of ~3.5 mm and the AB region highlighted in blue. Three trials made with similar results. d MIP image of the AB stained with BrpNc82 coupled with Cy3, with individual active zones highlighted in the top left part by surface rendering. e Enlarged 3D view of the identified active zones in the AB shown in (d), with different orientations of the T bar, the primary unit of active zone architecture (arrowheads). f Color-coded depth mapping of (d) to 30 µm with z-intervals of 10 µm. Single or multiple clusters in different orientations (arrowheads) are shown. The inset is a zoomed in view of the clusters marked by a red arrowhead. Note that the scale bars in real physical units (white font) reflect the 40x expansion factor (yellow font).

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