Fig. 4: Effect of pRS413-tRNA array on growth of synXVI.
From: Construction and iterative redesign of synXVI a 903 kb synthetic Saccharomyces cerevisiae chromosome
BY4741 and strain 20 harbouring synthetic megachunks A2 to BB4 were transformed with a pRS413 empty vector, and a pRS413 vector harbouring 17 tRNAs removed in the design of chromosome XVI (Strains 20 and 21, respectively). Strains demonstrated impaired growth on non-fermentable carbon sources at 37 ˚C. Introduction of the 17 tRNA genes shows improved fitness in the synthetic haploid strain compared to the vector-only control at 37 ˚C, which is especially pronounced on YP-Glycerol medium.
