Fig. 2: DEL1 - DEL8 inhibit 3CL protease in a biochemical assay and suppress SARS-CoV-2 viral replication in a cellular assay. | Nature Communications

Fig. 2: DEL1 - DEL8 inhibit 3CL protease in a biochemical assay and suppress SARS-CoV-2 viral replication in a cellular assay.

From: Development of small molecule non-covalent coronavirus 3CL protease inhibitors from DNA-encoded chemical library screening

Fig. 2

A The dose-dependent effects of DEL1 through DEL8 on the activity of 20 nM SARS-CoV-2 3CL protease were tested. The fluorogenic peptide MCA-AVLQSGFR-Lys(DNP)-Lys-NH2, corresponding to the nsp4/nsp5 cleavage site in the virus was applied as the substrate. Mean of 2 biological replicates is plotted. Source data are provided as a Source Data file. B Ability of DEL1 through DEL8 to inhibit SARS-CoV-2 viral infection in human Huh-7 cells, which were transduced to overexpress ACE2 receptor (Huh-7ACE2) before infection with SARS-CoV-2 virus (2019-nCoV/USA-WA1/2020). Data are plotted as the mean ± s.d., n = 3 biological replicates. Source data are provided as a Source Data file.

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