Fig. 5: Fab 3 binding region is masked in the rigid αβ TCR/CD3 complex.
From: Structural characterization of two γδ TCR/CD3 complexes
a Depiction of the Fab 3 binding site in a Fab 3 bound 9C2 γδTCR ECD structure. The Fab variable region is shown as cartoon, whereas the TCR ECD is shown as surface. The Fab 3 epitope is highlighted in yellow. b Bound Fab 3 was modeled onto the αβ TCR from PDB ID 8ES7 through alignment of the TCR β-chain to TCRγ. The Fab variable region is shown as a cartoon, whereas the TCR ECD is shown as surface view. Apparent steric clash between Fab 3 and CD3δ is highlighted in red. Expansion of Vδ1 γδ T-cells from human donor PBMCs following culture with Ab 3. T-cell purity (c, gated on live, CD45+ cells), and counts (d) were assessed on day 13. N = 1 for flow cytometry experiments.
