Fig. 1: Mutagenesis in hTERT RPE-1 p53-/- cells untreated and treated with 250 µM KBrO3 after 100 cell divisions. | Nature Communications

Fig. 1: Mutagenesis in hTERT RPE-1 p53-/- cells untreated and treated with 250 µM KBrO3 after 100 cell divisions.

From: Contributing factors to the oxidation-induced mutational landscape in human cells

Fig. 1

A Schematic of experimental conditions for mutation accumulation, clonal isolation, WGS, and mutation calling. Image created with BioRender.com and licensed for publication under agreement number TD26OVBCYI. B Number of SNVs and INDELs per genome in untreated (NT) and KBrO3-treated (KBrO3) cells. Circles indicate biologically independent genomes sequenced (n = 4). Horizontal bars are median values. (* indicates p-value = 0.0286 by two-sided Mann-Whitney U test comparing KBrO3-treated to untreated clones) C SNV and D INDEL mutation signatures from treated and untreated genomes. E De novo generated INDEL signature found in human cancers containing greater than 25% of total mutations attributed to SBS18. Total mutations involved in each de novo generated INDEL signature are listed in Supplementary Fig. 4.

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