Fig. 8: Light-driven one-carbon trophic growth of E. coli. | Nature Communications

Fig. 8: Light-driven one-carbon trophic growth of E. coli.

From: A new-to-nature photosynthesis system enhances utilization of one-carbon substrates in Escherichia coli

Fig. 8: Light-driven one-carbon trophic growth of E. coli.The alternative text for this image may have been generated using AI.

a Optimizing photosynthesis system to achieve light-driven one-carbon trophic growth. Abbreviation: hv, photon energy; e−, electron; NPM*, photosystem NuoK* + PufL + MgP; CO2, carbon dioxide; CH3OH, methanol; HCOOH, formate; ATP, adenosine triphosphate; NADH, nicotinamide adenine dinucleotide reduced; ADRP, artificial dark reaction pathway; C1, C1module; C3, C3 module; IM, inner membrance. b The evolution trajectory of strain TC-F3. The OD600 of representative evolved strains by gradually decreasing glucose concentration from 50 mM to 0 mM. The t1 and t2 were used to calculate the doubling time of strain TC-F3 according to the equation in Supplementary Fig. 52. c The consumption of methanol, formate, and NaHCO3 for strain TC-F3, respectively. d Labeling pattern of proteinogenic amino acids on feeding with NaH13CO3 + 13CH3OH + H13COOH and NaH13CO3 + 13CH3OH + H12COOH, respectively. Abbreviation: CO2, carbon dioxide; CH3OH, methanol; HCOOH, formate; DHA, dihydroxyacetone; MET, methylglyoxal; PYR, pyruvate; AcCoA, acetyl-CoA; CIT, citrate; ICIT, isocitrate; α-KG, α-ketoglutarate; SuCoA, succinyl-CoA; SUC, succinate; FUM, fumarate; MAL, malate; OAA, oxaloacetate; Ala, alanine; Ile, isoleucine; Val, valine; Leu, leucine; Thr, threonine; Glu, glutamate; Pro, proline; Arg, arginine. Values were shown as mean ± s.d. from three biological replicates (c, d). Source data are provided as a Source Data file.

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