Fig. 1: Simultaneous comparison of human reference and variant enhancer activities in developing mouse embryos.
From: Rapid and quantitative functional interrogation of human enhancer variant activity in live mice
a Schematic overview of the dual-enSERT-1 strategy and its potential readouts for different types of enhancer variants. b Transgenic targeting efficiencies of all dual-enSERT-1 constructs generated in this study. c Representative images of transgenic hZRSref-mCherry/hZRS404G>A-eGFP embryos at E11.5. A close-up of the hindlimb with separate and merged channels and an outline depicting the gain of enhancer activity (green channel) in the anterior domain are shown. A, anterior; P, posterior. d Plots quantifying fold-change (log2) difference in normalised reporter fluorescence between variant and reference hZRS alleles. Two-sided paired t tests vs. Heart: Forelimb ZPA, P = ns; Forelimb Anterior, P = 0.00072; Hindlimb ZPA, P = ns; Hindlimb Anterior, P = 9.48E-08. Data represented as mean ± SEM. Data points represent independent biological replicates (n = 4 embryos). Scale bars, 500 μm. Source data are provided as a Source Data file.
