Fig. 5: RNAPs stalling aggravated the occurrence of TRCs. | Nature Communications

Fig. 5: RNAPs stalling aggravated the occurrence of TRCs.

From: A synthetic methylotroph achieves accelerated cell growth by alleviating transcription-replication conflicts

Fig. 5: RNAPs stalling aggravated the occurrence of TRCs.

a EU-labeled RNA content determination. It is flow chart of the experimental procedure for EU-labeled nascent RNA. b The EU-labeled new RNA in strains FMX783, FMX783ΔGreA, FMX783ΔDksA, and FMX783ΔMfd captured by laser scanning confocal microscope. c Quantification of cell fluorescence intensity in EU-labeled RNA content using ImageJ. Three times each experiment was repeated independently with similar results. Source data are provided as a Source Data file.

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