Fig. 7: Genomic and transcriptome sequencing analysis of synthetic methylotrophs. | Nature Communications

Fig. 7: Genomic and transcriptome sequencing analysis of synthetic methylotrophs.

From: A synthetic methylotroph achieves accelerated cell growth by alleviating transcription-replication conflicts

Fig. 7: Genomic and transcriptome sequencing analysis of synthetic methylotrophs.

a Common mutations detected using genome sequencing from three strains. C1 represented to the starting strain FMX506, E1 represented to the evolved strain FMX783, and E2 referred to the evolved strain FMX892. Six mutated genes were identified, among which a DNA topoisomerase 1 inhibitor gene TOPAI was the key mutation. b Cell growth by introducing mutations to strain FMX783. The box plots showing the minima, maxima, center, bounds of box and whiskers and percentile. c Variation of gene TOPAI in three evolved strains. TOPAI gene existed in different forms in strains FMX506, FMX783 and FMX892. d The effect of TOPAI expression on cell growth of strain FMX783. e Transcriptional up-regulation levels of genes mdh, atoA, atoB, atoD, atoE, citC, pyk and zwf. The box plots showing the minima, maxima, center, bounds of box and whiskers and percentile. f mRNA levels of holD, mukB, cspE, YjhQ and TOPAI genes. Strain abbreviation: E1, FMX506; C1, FMX783; C2, FMX892. All (b, e, f), Data are presented as mean values ± SD, n = 3 independent biological experiments. Source data are provided as a Source Data file.

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