Table 1 Binding affinities, stoichiometries, and thermodynamic parameters for interactions between variants of FBF-2 and LST-1
From: A higher order PUF complex is central to regulation of C. elegans germline stem cells
FBF-2 variant | LST-1 variant a | wt PIM present | N (sites) b | KD,app(µM) b | ∆H (kJ/mol)b | ∆G (kJ/mol) | -T∆S (kJ/mol) | |
|---|---|---|---|---|---|---|---|---|
1 | RBD+CT | LST-119–98 | A+B | 0.50 ± 0.01 0.57 ± 0.04 | 1.1 ± 0.2 1.9 ± 0.9 | −61.9 ± 3.6 −45.1 ± 7.3 | −33.4 −32.1 | 28.5 13.0 |
2 | RBD+CT | LST-119–98(Am) | B | 1.02 ± 0.04 0.98 ± 0.02 | 2.8 ± 0.8 3.7 ± 0.5 | −47.9 ± 4.1 −48.3 ± 2.0 | −31.2 −30.5 | 16.7 17.8 |
3 | RBD+CT | LST-119–98(Bm) | A | 1.06 ± 0.25 0.89 ± 0.30 | 24.1 ± 15.3 46.2 ± 24.3 | −46.3 ± 21.9 −48.3 ± 21.5 | −25.9 −24.4 | 20.3 23.9 |
4 | RBD+CT | LST-119–98(AmBm) | none | – – | Not detected | – – | – – | – – |
5 | RBD | LST-119–98 | A+B | 0.46 ± 0.01 0.46 ± 0.01 | 0.48 ± 0.07 0.52 ± 0.07 | −139 ± 3.7 −157 ± 4.3 | −35.5 −35.3 | 104 122 |
6 | RBD | LST-119–98(Am) | B | 1.15 ± 0.01 1.05 ± 0.01 | 0.13 ± 0.01 0.12 ± 0.03 | −64.1 ± 0.7 −68.9 ± 1.8 | −38.7 −38.9 | 25.4 30.0 |
7 | RBD | LST-119–98(Bm) | A | 0.83 ± 0.02 0.86 ± 0.01 | 1.46 ± 0.25 1.54 ± 0.18 | −50.8 ± 1.8 −52.5 ± 1.2 | −32.8 −32.6 | 18.0 19.9 |
8 | RBD | LST-119–98(AmBm) | none | – – | Not detected | – – | – – | – – |
