Fig. 1: Molecular engineering of a PE-VLP system.

a Constructs involved for PE VLP production (left) and scheme of PE-VLP particles (right). b Evolutions of GAG-PE constructs (left) and efficiencies of VLPs produced with GAG-PE V1-V4 and a pegRNA (right). The protease site of MLV (P) and three fused Nuclear Export Signal of HIV-1 (3n) are indicated. (n = 3). c Structure of (e)pegRNAs targeting YFPs (left). The trimmed-EvopreQ1 structure (t-EvoQ1), MS2sl, TAR and PP7 hairpins are indicated. Created in BioRender. Ohlmann, T. (2024) https://BioRender.com/g43z718. (right) Relative performances of epegRNAs packaged within V4 PE-VLPs (n = 9). d Principle of the fidelity assay: HEK293T cells were modified by lentiviral transduction to carry a YFP stop cassette or cassettes harboring one or two mismatches relative to the epegT sequence. Sequences of YFPs variants are given with mismatched positions (red for 1 mismatch, purple for 2 mismatches) e Measurement of YFP conversion in cells edited by transfection (orange bars) or by PE-VLPs (blue bars). (n = 3). Data represent means of transduction in reporter cells +/− S.E.M. NTD: non-transduced conditions. For panels b c and e, n corresponds to independent transduction assays (biological replicates). Source data are provided as a Source Data file. Graphs were Created in BioRender. Ohlmann, T. (2024) https://BioRender.com/s01x964.