Fig. 2: Optimization of PE-VLPs performances using the PEmax evolution.
From: Delivery of Prime editing in human stem cells using pseudoviral NanoScribes particles
a Constructs encoding optimized editors (Cas9n*-RT*: PE Max) fused to GAG. Protease site (P in red) and Nuclear Export Signals from HIV-1 (3n) are indicated. b Compared efficiencies of V4-V12 PE-VLPs in transduced SWYS cells. NTD stands for non-transduced condition (n = 6). c Edition of endogenous locus by PE-VLPs in HEK293T cells (n = 5). Data show means +/− SEM. For panels b and c, n represent independent transduction assays (biological replicates). Source data are provided as a Source Data file. Graphs were Created in BioRender. Ohlmann, T. (2024) https://BioRender.com/s01x964.
