Fig. 3: Expression of pegRNA with a POL II-promoter enhances prime editing delivery.
From: Delivery of Prime editing in human stem cells using pseudoviral NanoScribes particles
a Structure of post-splicing ipegRNA and epegRNA. Created in BioRender. Ohlmann, T. (2024) https://BioRender.com/g43z718. b Efficacy of PE measured in SWYS cells transduced with GAG-PEV4-VLPs produced from cells expressing intronic-pegRNAs. Production was performed with addition of the Csy4 protein or its non-cleaving mutant Csy4H29A. (n = 9) c Comparison of GAG-PEV4 and GAG-PE-V7 for the production of ipeg-loaded VLPs. (n = 9). d Impact of ipegRNA on VLP-efficiencies on endogenous loci (HEK3 CTT insertion) or (RNF2 GTA insertion) (n = 3). e dose response of PE-VLP-efficiencies (HEK3 T > A substitution) in HEK293t cells (n = 3). For all panels, bars represent means +/- SEM and n independent transduction assays (Biological replicates). Source data are provided as a Source Data file. Graphs were Created in BioRender. Ohlmann, T. (2024) https://BioRender.com/s01x964.
