Fig. 7: PKA activity is regulated by CDK4 but is not sufficient to mediate apoptosis.

a Boxplot of kinome profiling/PAMgene analysis CDK4-WT and CDK4-KO TNBC cells. Comparison CDK4-KO vs. CDK4-WT TNBC cells. N = 4 independent biological replicates. Box spans from the first to the third quartile, marking the median with a distinct line. The whiskers reach out to the extreme maximum and minimum data point. b Immunoblots of Phospho-PKA substrates and MEM code in CDK4-WT and -KO TNBC cells. Relative quantity of Phospho-PKA substrates normalized to MEM code. Mean +/- SEM of N = 6 independent biological replicates. Two-sided Unpaired T-test. c Immunoblots of Phospho-PKA substrates and MEM code of CDK4-WT and -KO TNBC cells. Relative quantity of Phospho-PKA substrates relative to MEM code. Mean +/- SEM of N = 3 independent biological replicates. d Immunoblots of Phospho-PKA substrates and MEM code of CDK4-WT and -KO TNBC cells treated for 24 hours pretreatment with Forskolin (20 μM). e Relative cell number compared to control (no treatments) in CDK4-WT and -KO TNBC cells, upon 24 hours pretreatment and 3 consecutive days of Forskolin (20 μM). Cell death was induced in parallel with H₂0₂ (250μM-2hours) or Oligomycin and Antimycin (O + A) (1 + 10μM-2hours). Mean +/- SEM of N = 5 independent biological replicates. 2-way ANOVA; Tukey’s multiple comparison tests. Exact p-values are displayed in italic (bold italic if <0.05).