Fig. 2: Crol and hZNF267 control ISC proliferation through String/Cyclin B.
From: Steroid hormone-induced wingless ligands tune female intestinal size in Drosophila
A–D Confocal images showing R5 regions of MF midguts after seven days (7 d) of esgReDDM tracing showing (A) controls with active esg> in ISC/EB driving expression of >CD8::GFP (membrane GFP) and >H2B::RFP (nuclear RFP), (B) ISC/EB specific overexpression (OE) of crol (F003414) with (B’) showing anti-Discs large 1 (Dlg1) staining in septate junctions of EC, (C) >crol-RNAi (BL41669), and (D) simultaneous expression of >crol-RNAi (BL41669) and the human Crol orthologue ZNF267 (>hZNF267). Scale bar is 50 µm. E Quantification of progenitor numbers upon esgReDDM manipulations of crol (p = 0.0041; p = 0.0007; p = 0.011). F–I Confocal images of MF midguts after 7 d showing (F) ISC/EB specific OE of string (stg) (F000926), (G) combined with >crol (BL58359), and (H) specific KD of stg by RNAi (I) combined with >crol-RNAi (BL41669). J Quantitative RT-qPCR on midgut cDNA of MF fed with RH5849 showing relative mRNA levels of stg and Cyclin B (CycB) normalized to MeOH control (p = 0.0052; p = 0.0459). K, L Quantification of (K) ISC lineage production (ISC/EB and newly differentiated cells, p = 0.002; p = 0.0111; p = 0.0012; p = 0.0039), and (L) number of αPH3+ ISC in R5 upon combined manipulations of crol and stg. E, J, K, L For Box-and-whisker plots: the center is the median, minima and maxima are 25th and 75th quartile and whiskers indicate full range of values. All individual values with ‘n’ representing numbers of biological replicas are shown by dots and means are indicated by ‘+’. Asterisks denote significances from multiple comparisons by Kruskal-Wallis test (*p < 0.05; **p < 0.01; ***p < 0.001). Source data are provided as a Source Data file. M Schematic showing inhibition of Stg and CycB by Crol within ISC thereby controlling proliferation.
