Fig. 1: Sequence conservation and purification of ASFV RNAP.
From: Structural basis of RNA polymerase complexes in African swine fever virus
a Phylogenetic analysis of the two largest subunits of RNAP to determine evolutionary relationships. b ASFV RNAP was purified from cell extracts infected with ASFV CN/GS/2018-strep and fractionated on a sucrose gradient ranging from 10% to 30%. The respective subunit bands were visualized using silver staining on SDS-PAGE. This is a representative group of gels, which were repeated three times under the same condition with similar results. Source data are provided in a Source Data file. c The RNA polymerization activities using different template were assessed by measuring the incorporation of [α−32P]-ATP and normalized against the negative controls lacking RNA polymerase. The data represents the mean values ± standard deviation (error bars) from three independent experiments, with source data provided in a Source Data file. Statistical significance was determined using an unpaired two-tailed t-test, resulting in a P-value of 0.0003 with a 95% confidence interval (−8.491,−5.182) and degrees of freedom of 4. All calculations were conducted using Graphpad Prism 8 software.
