Fig. 4: G4 formation in hPKD1. | Nature Communications

Fig. 4: G4 formation in hPKD1.

From: G-quadruplex stabilization provokes DNA breaks in human PKD1, revealing a second hit mechanism for ADPKD

Fig. 4

BG4-ChIP of HEK293T or mIMCD3 chromatin from cells treated with vehicle (DMSO), 10 µM Phen-DC3 (left) or 0.1 µM CX-5461 (right). Primers specific for a region adjacent to human PKD1 IVS21 (G4-rich) or IVS34 (G4-poor), and mouse IVS21 or IVS37 (both G4-poor) were used in qPCR to determine enrichment. Locus amplification is displayed as 2−delta Ct. For Phen-DC3, data are presented as mean values ± s.e.m. (n = 6 from two independent experiments) *** = P < 0.001, P > 0.999 for IVS34, IVS21 (mouse), and IVS37. The experiment was repeated with another G4-ligand, CX-5461 (right), and data are presented as mean values ± s.e.m. (n = 6 from two independent experiments) *** = P < 0.001, P = 0.681 for IVS34, P = 0.959 for IVS21 (mouse), and P = 0.785 for IVS37. Source data provided as a Source Data file.

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