Fig. 6: G4 stabilization results in activation of the DNA damage response at PKD1. | Nature Communications

Fig. 6: G4 stabilization results in activation of the DNA damage response at PKD1.

From: G-quadruplex stabilization provokes DNA breaks in human PKD1, revealing a second hit mechanism for ADPKD

Fig. 6

a qPCR data for PKD1 and PCNA from ChIPs of genomic DNA with anti-γH2AX antibody in DMSO, Phen-DC3 (left) or CX-5461-treated (right) HEK293T and mIMCD3 cells. For both ligands, data are presented as mean ± s.e.m. (n = 6 from two independent experiments for each ligand (four total experiments/IPs)), two-way ANOVA, *** = P < 0.001. For Phen-DC3, hPCNA P = 0.784, mPkd1 P > 0.999, and mPcna P = 0.741. For CX-5461, hPCNA P = 0.623, mPkd1 P = 0.263, mPcna P = 0.947. b qPCR data for PKD1 and PCNA from ChIPs of genomic DNA precipitated with anti-RAD51 antibody in DMSO, Phen-DC3 (left) or CX5461-treated (right) HEK293T. Data are presented as mean ± s.e.m. (n = 6 from two (Phen-DC3) and n = 9 from three (CX-5461) independent experiments), two-way ANOVA, *** = P < 0.001. For Phen-DC3 hPCNA P = 0.431, for CX-5461 hPCNA P = 0.931. Amplification results for ChIPs are displayed as 2−delta Ct. Source data provided as a Source Data file.

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