Fig. 1: Tasl^–/– mice show defective response to toll-like receptor 9 and 7 agonists.

a Serum IFN-α levels from Tasl^–/– (KO) and wild type (WT) littermate mice injected with either LPS (10 mg/kg), R848 (10 μg), or CpG A (100 μg). n = 5 mice for all treatment groups except WT mice treated with LPS (n = 4). b Splenic plasmacytoid dendritic cells (pDCs), B cells, and neutrophils were isolated from Tasl^–/– (KO) and wild type (WT) littermate mice and stimulated with either LPS, R848, or CpG A. Cytokines were measured from supernatants either 24 h post stimulation (pDCs, neutrophils) or 48 h post stimulation (B cells). c Bone marrow derived Flt3L pDCs were stimulated with either poly I:C (50μg/ml), LPS (5 μg/ml), R848 (10 μg/ml), CpG A (10μg/ml), interferon stimulatory DNA ISD (4 μg/ml), or 5’pdsRNA(2 μg/ml) and IL-6 levels was measured from supernatants 24 h post stimulation. d RT-qPCR analysis of Type I IFN genes of WT and KO Flt3L pDCs stimulated with CpG A (1 μg/ml) for the indicated times. All data are means ± SEM and are representative of at least 2 independent experiments. Cells were isolated from 5 mice per genotype that were pooled. In (a), (b), Unpaired t test (2-tailed) *** P < 0.001, ** P < 0.005 In (c) and (d), Two-way ANOVA ***P < 0.0001; ** P < 0.005, *P < 0.05.