Fig. 4: roX RNAs directly repress autosomal genes involved in female development.

a Scatter plot showing expression changes of roX-bound autosomal genes after roX loss and sex ratio in their expression (female versus male). Pearson’s correlation coefficients (R) were used to assess these associations. The linear regression line is depicted in black, with the 95% confidence interval represented as a lightgrey shadow. b A majority of autosomal genes directly repressed by roX show female-biased expression. The center lines represent the median, the box limits represent the first and third quartiles, and the whiskers indicate 1.5 × the interquartile range (IQR). c roX directly repressed autosomal genes showing female-biased expression are associated with higher H3K27me3 levels in males on roX-binding sites, which declined after roX loss. d Enriched GO terms of roX directly repressed, female-biased autosomal genes as shown in (c). The terms related to female development are highlighted in red. Significance levels are determined using the one-sided hypergeometric test. Source data are provided as a Source Data file.