Fig. 2: Actin is mainly associated with tip-to-side mitochondrial fusion.

a Schematic representation of tip-to-tip and tip-to-side fusion events. b, c Quantification of fusion events as end to-side (Side) or tip-to-tip (End) (b), and their corresponding association with AC-mito (c) in cells from Fig. 1b, c (transfected with mCherry-mito and AC-mito). Each point represents an individual cell, with 10 cells (e) quantified in 4 independent experiments. Bars show the average ± SD. Two-sided t-test. Total events from the quantified cells are shown in (c). d, e Quantification of fusion events containing one or two mobile mitochondria (d) and their corresponding association with AC-mito (e). Each point represents an individual cell with 8 cells quantified in 4 independent experiments. Bars show the average ± SD. Two-sided t-test. Total events from the quantified cells are shown in (e). f Quantification of AC-mito signal associated with each of the fusing mitochondria (the stationary mitochondrion (Stat) and the mobile mitochondrion (Mobile)) as well as with the fusion site one frame post-fusion (Post). Each point represents an individual cell, with 9 cells quantified in 4 independent experiments. Bars show the average ± SD. One-way ANOVA.