Fig. 4: Mitochondrial actin is required for both fission and fusion.
From: Mitochondria- and ER-associated actin are required for mitochondrial fusion
a Representative images showing the loss of AC-mito signal (green) in cells transfected with DeAct-mito or DeAct-ER (magenta) and AC-mito. b, c Quantification of AC-mito (b) and AC-ER (c) signal accumulation in cells transfected as in (a). Each point represents an individual cell, with 14 (mCh), 10 (DeAct mito; b), 16 (DeAct mito; c), 22 (DeActER; b) and 14 (DeActER; c) cells quantified in 3 independent experiments for each condition. Bars show the average ± SD. One-way ANOVA. d Quantification of the number of fission (Left) and fusion (Right) events in cells transfected with the indicated DeAct probe, AC-mito and mCherry-Fis1 (to label mitochondria). Each point represents an individual cell, with 11 (mCh), 8 (DeAct-mito) and 9 (DeAct-ER) cells quantified in 3 independent experiments. Bars show the average ± SD. One-way ANOVA. e Quantification of mitochondrial length (Left) and connectivity (Right) in cells from (d). Each point represents an individual cell. Bars show the average ± SD. One-way ANOVA. f fusion/fission ratio quantified from the data in (d). g Quantification of fusion events as tip-to-side (Side) or tip-to-tip (End). The total number of events for 8 cells in 3 experiments is shown for each condition. Chi-square test.
