Fig. 6: Formin-dependent regulation of mitochondrial fusion.

a Representative images showing AC-mito signal in primary fibroblasts transfected with AC-mito (green) and mCherry-Fis1 (magenta), and treated with the formin inhibitor SMIFH2. Scale bar 10 µm (b) Quantification of AC-mito signal accumulation in cells transfected as in (a). Each point represents an individual cell, with 12 cells quantified in 3 independent experiments. Bars show the average ±SD. Two-sided t-test. c, d Quantification of the number of fusion (c, Left, blue) and fission (c, Right, orange) events, as well as the fusion/fission ratio (d) in cells transfected as in (a) and treated as indicated. Each point represents an individual cell, with 12 cells quantified in 3 independent experiments. Bars show the average ± SD. Two-sided t-test. e Quantification of mitochondrial length (Left) and connectivity (Right) in cells from (b). Each point represents an individual cell. Bars show the average ± SD. Two-sided t-test. f Quantification of fusion events as tip-to-side (Side) or tip-to-tip (End). The total number of events for 12 cells in 3 experiments is shown for each condition. g, h Quantification of the number of fusion (Left, blue) and fission (Right, orange) events, as well as the fusion/fission ratio (h) in U2OS cells in which INF2 has been deleted using CRISPR/Cas9. Each point represents an individual cell, with 12 cells quantified in 3 independent experiments. Bars show the average ± SD. Two-sided t-test. i Quantification of fusion events as tip-to-side (Side) or tip-to-tip (End). The total number of events for 12 cells in 3 experiments is shown for each condition.