Fig. 5: The electron microscopy structure of the hOCCM. | Nature Communications

Fig. 5: The electron microscopy structure of the hOCCM.

From: Reconstitution of human DNA licensing and the structural and functional analysis of key intermediates

Fig. 5

a Negative stain class averages of a low-salt washed pre-RC reaction containing ATPyS. We observed the following pre-RC complexes: single hMCM2-7 hexamers, hOCCM and hMCM2-7 double hexamers. The green densities represent hMCM2-7 rings, shaded for orientation with light green highlighting the C-terminus and darker green N-terminus. hORC-hCDC6 rings have been shaded with pink. b Representative 2D class averages from cryo-EM of the hOCCM denoting top and side views (upper), and projections of the resulting 3D map onto the 2D class averages showing good fit (lower). c The segmented experimental density map is shown from different angles and coloured by protein subunit. d The hOCCM molecular model was built from the cryo-EM structure (PDB: 8RWV). e Structure comparison of hOCCM (in colours) with yOCCM (grey, PDB: 5V8F).

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