Fig. 8: DNA structure and interactions in hOCCM.
a The DNA interacting with yORC in the yOCCM (PDB: 5V8F; grey) is bent by 10Ā° respect to that of the hOCCM (orange). The hOCCM DNA is stretched with respect to the canonical B-form DNA (light grey) in the upper part of the DNA. b Detailed hORC1-5 and hCDC6 interactions with DNA. The upper deformed part of the DNA is contacted by the ISM, and the lower less deformed part by WHDs. The view is rotated 180Ā° with respect to (a). c hORC4-DNA interactions. hORC4 (light blue) is positioned closer to the DNA in hOCCM than in yOCCM (PDB: 58VF; grey). The DNA is not well-resolved enough in hORC1-5 (PDB: 7JPS; light grey). d hORC4 sequence alignment. The ISM loop region is longer in yeast containing a helix insert, while higher eukaryotes contain a conserved alternative ISM sequence. e Alternative Orc4 WHD close interactions with DNA in the human (blue) and yeast (grey) proteins.
