Fig. 4: Pseudotime analysis of all cotton ovules samples from −1.5-DPA to 1-DPA.

a A pseudotime trajectory shows the distribution of cell clusters. The dashed arrow indicates the developmental direction of the pseudotime trajectory. b Proportion of pseudotime states in the four cotton ovule samples. c Pseudotime trajectories colored according to tissue type. d Heatmap clustering of key genes for developmental trajectories in all samples and expression kinetics of genes over pseudotime. The names of genes and cell types that determine the developmental trajectories are shown on the right side of the heatmap. Expression levels are indicated by the color ranging from blue (low) to red (high). Source data are provided as a Source Data file.