Fig. 2: BA.2.86 and JN.1 efficiently evade neutralization in double boostered individuals, but the adapted XBB.1.5 vaccine booster enhances protection against both.
A Particles pseudo-typed with the indicated S proteins were pre-incubated for one hour at 37 °C with plasma dilutions from double boostered health care workers (biological replicates n = 30) (2A) or with plasma dilutions following vaccination with an adapted XBB.1.5 booster (biological replicates n = 30) (2B). Pseudo-virus neutralization titer 50 (PVNT50) was calculated using the least squares fit using a variable slope, using a four-parameter nonlinear regression model and values were plotted as geometric mean. Geometric mean standard deviation bars are shown in black. The lower limit of confidence (LLOC) was set at a PVNT50 of 50. Non responders are defined as individuals below 60 (dashed line). All PVNT50 below 10 are set at 10 for visualization purposes. The assay was performed in technical duplicates and with negative controls to assess the virus input of each used pseudo-virus in the absence of plasma antibodies. Percentage of positive responders, geometric means, and fold change neutralization over WT-Wuhanpp are shown on top. Friedman two sided non parametric paired test (ns, p > 0.05; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001). Percentage of positive responders, geometric means, and fold change neutralization over WT-Wuhanpp are shown on top. Source data are provided as a Source Data file.
