Fig. 3: Mapping mutations in the NTD for effects of BA.2.86 neutralization efficiency in double boostered individuals.
A Neutralization assessment for particles pseudo-typed with mutations within the NTD of BA.2.86. Each mutant shown contains a single mutation reverting the amino acid mutation in BA.2.86 to the corresponding amino acid within BA.2. Particles pseudo-typed with the indicated S proteins were preincubated for one hour at 37 °C with plasma dilutions from double boostered health care workers with non-adapted immunogens. The numbers of biological replicates corresponding to individual plasma samples are shown on top. Pseudo-virus neutralization titer 50 (PVNT50) was calculated using the least squares fit using a variable slope, using a four-parameter nonlinear regression model. The lower limit of confidence (LLOC) was set at a PVNT50 of 50. Non responders are defined as individuals below 60 (dashed line) All PVNT50 below 10 are set at 10 for visualization purposes. The assay was performed in technical duplicates and with negative controls to assess the virus input of each used pseudo-virus in the absence of plasma antibodies. Percentage of positive responders, geometric means, and fold change neutralization over WT-Wuhanpp are shown on top. Geometric mean standard deviation bars are shown in black. B Individual neutralization data for particles pseudo-typed with mutations within the NTD of BA.2.86. Statistical significance was assessed by Friedman two sided non parametric paired test (ns, p > 0.05; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001). Source data are provided as a Source Data file.
