Fig. 1: Genome-wide CRISPR screen identifies GGCX as EA H1N1 SIV host-dependent factor in porcine kidney cells.
a A schematic diagram of the genome-wide CRISPR screening process in PK-15 cells. b–e Effects of GGCX-KO on the replication of influenza virus strains namely, (b) HuB/H1N1, (c) HuN/H1N1, (d) PR8/H1N1, and (e) SH13/H9N2. GGCX-KO and WT PK-15 cells were infected with IAV strains at a MOI of 0.1, and the viral titers at indicated time points were determined by TCID50. SLC35A1 is an IAV host-dependent factor, and SLC35A1-KO cells were used as a positive control. f Restoration of GGCX promotes influenza virus replication. Exogenous GGCX (Flag-GGCX) and Flag negative control were transfected into GGCX-KO PK-15 cells or WT PK-15 cells. The transfected cells were infected with HuB/H1N1 at an MOI of 0.1, viral titers at 24 hpi were determined by TCID50, and corresponding protein expression was detected by western blot. The error bar in panels (b–f) indicates the standard deviation. The data shown in panels (b–f) are means ± SD (n = 3 biologically independent experiments). Statistical analysis was performed using an unpaired, two-tailed Student’s t test. (ns, P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001). The statistical analysis in panel (b) is between the GGCX-KO and Control. Exact P-values are available in the Source Data. Source data are provided as a Source Data file.
