Fig. 4: Osteoblast-specific Gpx4 knockout or knockdown suppresses osteoblastogenesis.

a Representative micro-CT images of the three-dimensional long bone, trabecular bone, and cortical bone in femur of Gpx4^OBs−/− mice and Gpx4^flox/flox littermates. Scale bar = 1 mm. b Micro-CT measurements of Ct.BMD, Ct. BV/TV, Ct.Th, and BV/TV of trabecular bone, Tb.Th as well as Tb.N at the distal femur from Gpx4^OBs−/− mice and Gpx4^flox/flox mice (n = 6 independently biological samples). Ct.BMD, bone mass density of cortical bone. Ct. BV/TV, ratio of bone volume to total volume of cortical bone. Ct.Th, thickness of cortical bone. Trabecular bone BV/TV, ratio of bone volume to total volume of trabecular bone. Tb.Th, trabecular thickness. Tb.N, trabecular number. c Representative H&E staining images of femurs from Gpx4^OBs−/− mice and Gpx4^flox/flox mice. Scale bar = 100 μm. d Representative von Kossa staining images of femurs from Gpx4^OBs−/− mice and Gpx4^flox/flox mice. Scale bar = 1 mm. e–f Representative ALP immunohistochemical images (e) and quantification (f) of femur from Gpx4^OBs−/− mice and Gpx4^flox/flox mice. Scale bar = 20 μm. n = 4 (Gpx4^flox/flox mice group) or 5 (Gpx4^OBs−/− mice group) biologically independent samples. g qPCR analysis of genes expression related to bone formation (n = 5 independently biological samples). The tissues were harvested from Gpx4^OBs−/− and Gpx4^flox/flox mice at two months of age. h ALP staining in 1 week of osteogenic induction and alizarin red staining of mineralized matrix in MC3T3-E1 cells after 3 weeks of osteogenic induction post knockdown of GPX4. i ALP staining in osteoblasts after 1 week of osteogenic induction and alizarin red staining of mineralized matrix in MC3T3-E1 cells after 3 weeks of osteogenic induction treated with GPX4 siRNA and ferrostatin-1 (Fer-1, 5 μM). Data are presented as means ± SD. Statistical analysis was performed with unpaired two-tailed t-test. Source data are provided as a Source Data file.