Fig. 5: Single-cell metabolic profiling of RDEB T and NK cells.

a Schematic presentation of single-cell energetic metabolism by profiling translation inhibition (SCENITH). Visualization of protein synthesis (PS) after puromycin incorporation by flow cytometry. Histograms display the level of PS in control (Co) and in the presence of specific metabolic inhibitors (DG, O or DGO). b–d Single-cell metabolic profile presented as scatter plots displaying the translation level, Fatty acid Amino acid oxidation (FAAO), glycolytic capacity, glucose dependence and mitochondrial dependence in CD4⁺, CD8⁺ cells and NK cells. Healthy controls (n = 6, black triangles) and RDEB adults (n = 6, red circles). All results are presented in scattered plots as mean values from 6 independent donors ± SEM. Statistical analyses were performed with two-sided unpaired t-test. Asterisks represent significant differences between healthy controls and RDEB adults (*p < 0.05, **p < 0.01). Source data are provided as a Source Data file.