Fig. 3: PRDX1 knockout increases NASH and liver fibrosis. | Nature Communications

Fig. 3: PRDX1 knockout increases NASH and liver fibrosis.

From: Inhibited peroxidase activity of peroxiredoxin 1 by palmitic acid exacerbates nonalcoholic steatohepatitis in male mice

Fig. 3: PRDX1 knockout increases NASH and liver fibrosis.The alternative text for this image may have been generated using AI.

a Representative images validating the efficiency of PRDX1 knockout in Prdx1-/- mice. This experiment was repeated for three times independently. b Peroxidase activity of global hepatic PRDX in WT and Prdx1-/- mice. WT mice (n = 9); Prdx1-/- mice (n = 8). c Body weight of WT and Prdx1-/- mice on WD. 8-week-old male mice were fed a WD for 20 weeks and their weekly body weights were monitored. n = 10 mice per group. d Daily food intake of mice on WD (as in c). n = 10 mice per group; ns, no significance. e Intraperitoneal glucose tolerance test (IPGTT) in mice on WD (as in c) and area under the curve (AUC). n = 10 mice per group. f Intraperitoneal insulin tolerance test (IPITT) in mice on WD (as in c) and AUC. n = 10 mice per group. g Circulating ALT and AST levels (as in c). WT mice (n = 8); Prdx1-/- mice (n = 10). h Representative images of HKperox-Red staining in the liver and quantitative analysis (as in c). Arrows denote the signals of HKPerox-Red staining. Scale bar, 50 μm. n = 9 images from three mice per group. i Representative images showing H&E and Oil Red O staining in the liver after WD (as in c). n = 3 biologically independent mice. Scale bars, 50 μm. j Representative images showing Sirius Red and α-SMA staining in the liver (as in c). n = 3 biologically independent mice. Scale bars, 50 μm. All data are presented as means ± SEM. Unpaired and two-tailed Student’s t test was performed for b, d, AUC of e, AUC of f, g, and h. Two-way ANOVA followed by Bonferroni’s test for multiple comparisons was performed for c, e, and f.

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