Fig. 2: DDM binding pocket of SPNS2.
From: Transport and inhibition of the sphingosine-1-phosphate exporter SPNS2
a Bound n-dodecyl-β-D-maltopyranoside within the SPNS2 structure determined in DDM. Positive difference density of the weighted Fo − Fc difference map at 16σ and the DDM model are shown as blue surface and DDM cyan sticks, respectively. b Cross section of the SPNS2 structure, viewed from the plane of the membrane. c SPNS2 coordinates DDM through van der Waals contacts in the pocket and hydrogen bonds in the central cavity. SPNS2-DDM hydrogen bonds are shown as dotted lines. d SPNS2 N-domain contains a patch of polar residues adjacent to the DDM head group. e Schematic for GPCR-based transport assay. Media from cells expressing SPNS2 and SphK1 is collected and applied to reporter cells expressing S1PR3 and the Ca2+ biosensor obelin. f Measurement of S1P export activity by SPNS2 mutants (N = 24 independently treated samples). Data are presented as mean ± SEM.
