Fig. 5: LEC-derived 25-HC modulates tumor growth by impacting tumor-associated macrophages and monocytes.
From: Lymphatic-derived oxysterols promote anti-tumor immunity and response to immunotherapy in melanoma
A LECΔCh25h and LECWT mice were inoculated with B16F10-OVA VEGF-C cells and vaccinated with OVA protein and CpG-B at day 4 and day 8. Mice were additionally injected with anti-CSF1R (1 mg / mouse at day 10, followed by 500 µg/mouse every 3 days or PBS), as represented in the scheme. Tumor growth was followed. Two-way ANOVA, **P < 0.01. Data were representative of two independent experiments with n = 5–7 mice/group each. B NSG-LECΔCh25h and NSG-LECWT mice were inoculated with B16F10-OVA VEGF-C cells mixed or not with BMDM. Tumor-bearing mice were further adoptively transferred with OT-1 effectors on day 8, as represented in the scheme. Tumor growth was followed and normalized to the size individually from day 6. Two-way ANOVA, **P < 0.01. Data were pooled from two independent experiments with n = 5–8 mice/group each. A, B Data were presented as mean values ± SEM.
