Fig. 4: Transcription analysis of the tmRNA–smpB ribosome-rescue system in S. albus J1074 harbouring the wild-type or split busA genes. | Nature Communications

Fig. 4: Transcription analysis of the tmRNA–smpB ribosome-rescue system in S. albus J1074 harbouring the wild-type or split busA genes.

From: Improving polyketide biosynthesis by rescuing the translation of truncated mRNAs into functional polyketide synthase subunits

Fig. 4: Transcription analysis of the tmRNA–smpB ribosome-rescue system in S. albus J1074 harbouring the wild-type or split busA genes.The alternative text for this image may have been generated using AI.

a qRT‒PCR transcription analysis of the ssrA gene (encoding the tmRNA in the tmRNA–smpB ribosome-rescue system) in J1074 strains harbouring butenyl-spinosyn gene clusters containing the wild-type or split busA genes indicated in Fig. 1b. b qRT‒PCR analysis of the smpB gene of the tmRNA–smpB ribosome rescue system in J1074 strains harbouring butenyl-spinosyn gene clusters containing the wild-type or split busA genes. n = 3 independent biological samples. Data are presented as the mean ± S.D. The p-values of the two-tailed t test are indicated. ns, not significant. Source data are provided as a Source Data file.

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