Fig. 5: mRNA truncation increases production rate of PKS proteins encoded by genes closer to the operon promoter. | Nature Communications

Fig. 5: mRNA truncation increases production rate of PKS proteins encoded by genes closer to the operon promoter.

From: Improving polyketide biosynthesis by rescuing the translation of truncated mRNAs into functional polyketide synthase subunits

Fig. 5: mRNA truncation increases production rate of PKS proteins encoded by genes closer to the operon promoter.The alternative text for this image may have been generated using AI.

a Schematics of the wild-type and split busA genes fused with the gusA gene under the control of the cumate-inducible promoter. b Real-time GusA (β-glucuronidase reporter) activity assay of J1074 strains containing the DNA constructs from (a). c Production rate of BusA-1-1-GusA and BusA-2-1-GusA proteins in J1074 strains after induction for 40–80 min. d Production rate of BusA-GusA, BusA-1-1-GusA, and BusA-2-1-GusA proteins in J1074 strains after induction for 80–160 min. e β-Glucuronidase activity assay of J1074 strains containing the DNA constructs from (a) after induction for 20 h. f Schematics of the wild-type and split busA genes fused with the gusA gene under the control of the cumate-inducible promoter. g Real-time GusA (the β-glucuronidase) activity of J1074 strains containing the DNA constructs from (f). h Production rate of BusA-1-2-GusA and BusA-2-2-GusA proteins in J1074 after induction for 20–30 min. i Production rate of BusA-GusA, BusA-1-2-GusA, and BusA-2-2-GusA proteins in J1074 strains after induction for 60–160 min. j β-Glucuronidase activity of J1074 strains expressing the DNA constructs from (a) after induction for 20 h. n = 3 independent biological samples. Data are presented as the mean ± S.D. The p-values of the two-tailed t test are indicated. ns, not significant. Source data are provided as a Source Data file.

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