Fig. 6: Transcriptional reinforcement of downstream PKS genes further increases butenyl-spinosyn production. | Nature Communications

Fig. 6: Transcriptional reinforcement of downstream PKS genes further increases butenyl-spinosyn production.

From: Improving polyketide biosynthesis by rescuing the translation of truncated mRNAs into functional polyketide synthase subunits

Fig. 6: Transcriptional reinforcement of downstream PKS genes further increases butenyl-spinosyn production.The alternative text for this image may have been generated using AI.

a Schematic of the kasOp* promoter insertion. b–d Butenyl-spinosyn production by S. albus J1074 strains harbouring gene clusters from (a). n = 3 independent fermentation samples. e qRT‒PCR analysis of the transcription of bus genes in J1074 strains harbouring the busA-3 butenyl-spinosyn gene cluster with the kasOp* promoter inserted upstream of busB, busC, busD, or busE. n = 3 independent biological samples. Data are presented as the mean ± S.D. The p-values of the two-tailed t test are indicated. Source data are provided as a Source Data file.

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