Fig. 7: Functional rescue of visual neuronal responses can be attained with remyelination below healthy levels.

A Vehicle and low dose LL-341070 improve single-neuron latency to flash (dark and bright) from 3-7 weeks. High dose LL-341070 already recovers by 3 weeks. B Low and high dose LL-341070 restore single-neuron latencies to flash (dark and bright) by 7 weeks. C, D No differences between groups in VEP N70 latency (C) or shape (D) to flash (dark and bright) at 7 weeks. E Recovery of oligodendrocytes, myelin, and single-neuron responses with time. In (A) vehicle, Wilcoxon (Z = −2.3, *p = 0.022). 3w: n = 157 responses from 8 probes from 3 mice, 7w: n = 144 responses from 11 probes from 4 mice. In (A) 0.1 mg/kg, Wilcoxon (Z = 4.05, ***p < 0.0001). 3w: n = 40 responses from 6 probes from 4 mice, 7w: n = 77 responses from 8 probes from 3 mice. In (A) 0.3 mg/kg, Wilcoxon (Z = 1.11, p = 0.27). 3w: n = 76 responses from 4 probes from 2 mice, 7w: n = 32 responses from 3 probes from 1 mouse. In (B), Kruskal-Wallis (F(3) = 10.85, *p = 0.013). Steel-Dwass (vehicle vs. healthy: *p = 0.013). Vehicle: n = 144 responses from 11 probes from 4 mice, 0.1 mg/kg: n = 77 responses from 8 probes from 3 mice, 0.3 mg/kg: n = 32 responses from 3 probes from 1 mouse, healthy: n = 104 responses from 6 probes from 3 mice. In (C), Kruskal-Wallis (F(3) = 3.32, p = 0.34). Vehicle: n = 17 VEPS from 9 probes from 3 mice, 0.1 mg/kg: n = 27 VEPs from 14 probes from 5 mice, 0.3 mg/kg: n = 13 VEPs from 9 probes from 3 mice, healthy: n = 6 VEPs from 3 probes from 2 mice. In (D), Kruskal-Wallis (F(3) = 13.22, p = 0.0042). Steel-Dwass (vehicle vs. healthy: *p = 0.043; 0.1 mg/kg vs. healthy: *p = 0.012; 0.3 mg/kg vs. healthy: *p = 0.023). Vehicle: n = 17 VEPs from 9 probes from 3 mice, 0.1 mg/kg: n = 27 VEPs from 14 probes from 5 mice, 0.3 mg/kg: n = 13 VEPs from 9 probes from 3 mice, healthy: n = 6 VEPs from 3 probes from 2 mice. n.s. not significant, *p < 0.05, ***p < 0.001; median, IQR (box), min./max. (whiskers) in (A−D); data points color-coded by mouse in A-D; two-sided statistical tests. See Supplementary Data 1 for statistical details and Source Data file for source data. VEP=visual evoked potential.